Add Publication

Detection of Newcastle disease virus of poultry by real time reverse transcription-polymerase chain reaction

Publication Date - 2016-07-07 00:00:00

Publication Title : Detection of Newcastle disease virus of poultry by real time reverse transcription-polymerase chain reaction

Publicationed By : Md. Mostafijur Rahman

Publication Publication Date : 2016-07-07 00:00:00

Publication Online Link :

Publication Description :

A real-time reverse transcription - polymerase chain reaction (rRT-PCR) was used for the  detection of Newcastle disease virus (NDV) of poultry. A panel of seven known isolates of  NDV in the form of allantoic fluid, obtained from a laboratory repository, was used for the  development of the test. RNA was extracted from the allantoic fluid with a magnetic  processor based automated RNA extraction system. The identity of the reference virus  was first reconfirmed by a conventional RT-PCR specific for the Fusion (F) protein gene.    Using these RNA, the rRT-PCR protocol was optimized with regard to the reaction mix   and thermal profile using published primers and probes specific for M gene. The  sensitivity of standardized rRT-PCR was compared to that of the conventional RT-PCR  using serial 10-fold dilutions of the RNA of a selected sample. The thermal profile was  modified from the published one; the annealing and extension steps were combined to a  single step performed at 60ºC. The adopted rRT-PCR successfully amplified M gene from  all the seven reference samples with a C   value ranging from 15.28 to 32.68. The rRT-PCR  for M gene was 100-fold more sensitive than the conventional RT-PCR for F gene. This is  the first report of the use of rRT-PCR for the detection of NDV in Bangladesh. This test  will be useful for virological surveillance, particularly for screening NDV in respiratory  infections. (Bangl. vet. 2016. Vol. 33, No. 1, 16 – 22) 

Download File

Recent Publications

Stream Cipher Based Modified Espresso Algorithm for 5G Communication
Expeditious prediction of post-mortem changes in frozen fish meat using three-dimensional fluorescence fingerprints.
Visualize the quality of frozen fish using fluorescence imaging aided with excitation-emission matrix
Smart technique for accurate monitoring of ATP content in frozen fish fillets using fluorescence fingerprint
Potential Contribution of Fish Feed and Phytoplankton to the Content of Volatile Terpenes in Cultured Pangasius and Tilapia
Effect of porcine uterus as ex vivo model of fertilizing ability and gene expression pattern on blastocysts
Effects of pH on the Fluorescence Fingerprint of ATP
Effect of probiotic and sand filtration treatments on water quality and growth of tilapia and pangas in earthen ponds of southern Bangladesh
The Status of Geography and Environmental Studies at Secondary Level Education in Bangladesh
Assessment of Species Specificity of Fishing Gears and Fish Diversity Status in the Andharmanik River of Coastal Bangladesh