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Effect of porcine uterus as ex vivo model of fertilizing ability and gene expression pattern on blastocysts

Publication Date - 2019-02-26 00:00:00

Publication Title : Effect of porcine uterus as ex vivo model of fertilizing ability and gene expression pattern on blastocysts

Publicationed By : Dr. Dibyendu Biswas

Publication Publication Date : 2019-02-26 00:00:00

Publication Online Link : https://www.sciencedirect.com/science/article/pii/S0093691X18309579

Publication Description :

The success of in vitro embryo production demonstrates that the oviduct can be bypassed during early embryonic development. Using an ex vivo model of porcine uterus is one of the strategies used to investigate fertilization within the oviductal environment. In this study, in vitro-matured porcine oocytes (MII) were fertilized with 7.5  107, 15  107, or 30 107 sperm cells for 20 min in the oviduct of a porcine uterine ex vivo model. MII oocytes used for in vitro fertilization (IVF) served as control 1; those cultured in the oviduct of the ex vivo model for 20 min before IVF served as control 2. In present study, the penetration rate, polyspermy, and fertilization efficiency, and accumulated reactive oxygen species (ROS) levels in the treatment groups were significantly decreased compared to those in the control 1 group. During embryonic development, the cleavage rates in the treatment groups were significantly lower than those in the control groups. The cleavage rate in the 30  107 sperm cell-treated group was higher than that in the 7.5  107 sperm cell-treated group. The blastocyst formation rate in control 1 and 2, and 30  107 sperm cell-treated groups increased compared to that in the 7.5 and 15  107 sperm cell-treated groups. PCNA, HSP70.2, and GLUT1 were upregulated in the treatment groups and POU5F1, BAX, GPX1 were upregulated in the treatment and control 2 groups, compared to the control 1 group. These results suggest that an ex vivo model may decrease the penetration rate and fertilization efficiency by increasing the accumulated ROS levels and inducing the expression of apoptosis- and stress-related genes. However, the model improved the monospermy rate and expression of embryo developmental competence genes. This is the first study that evaluates the effect of an ex vivo model of porcine uterus on fertilization parameters, and the development of porcine embryos



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