Publication Title : Molecular characterization of Enterobacter and Escherichia coli pathotypes prevalent in the popular street foods of Dhaka city
Publicationed By : Md. Belal Hossain
Publication Publication Date : 2017-12-01 00:00:00
Publication Online Link : http://www.bsm.org.bd/journal1.php
Publication Description :
Food borne pathogenic enteric bacteria are of great concern for global public health. Among them, Escherichia coli and Enterobacter spp. are the most prevalent in the street food. In this study, we have isolated 23 strains of such enteric bacteria from multiple food samples by conventional cultural technique. Isolated strains were characterized molecularly into different genotypes using RAPD, amplified ribosomal DNA restriction analysis, and partial sequencing of 16S rDNA. RAPD represents 10 different types of strains whereas ARDRA clusters them into two separate groups. 16 out of the 23 isolates were identified as E. coli and the rest were as Enterobacter spp. by biochemical tests and were further confirmed by partial 16S rDNA sequencing. Significant level of virulence traits including stx1, stx2 and escV genes were identified in the E. coli strains. Also, most of the isolates were found resistant to azithromycin and amoxicillin. This study revealed the presence of various pathogenic enteric bacteria in various street foods with multidrug resistance. Therefore, this study suggests that people consuming such street foods are at major risk of food borne illness. Further studies are warranted to analyze their detailed pathogenicity of these isolates.